Complete Protocol

To start, you need a strain that is ade2^- (the ade2 deletion must be done with the appropriate primers #6 and #7 below[1]) and ura3^-. Alternatively you can use the strain ByK352.

The complete protocol start with Library Generation, then DNA preparation, then Sequencing, then Bioinformatics analysis.

primers

6 Ade2PriFwd GTATAAATTGGTGCGTAAAATCGTTGGATCTCTCTTCTAAcggatccccgggttaattaa

7 Ade2PriRev TATGTATGAAGTCCACATTTGATGTAATCATAACAAAGCCgaattcgagctcgtttaaac