pBK257

LOCUS pBK257 10888 bp ds-DNA circular 04-AUG-2016

DEFINITION .

ACCESSION

VERSION

SOURCE .

ORGANISM .

COMMENT

COMMENT

COMMENT ApEinfo:methylated:1

FEATURES Location/Qualifiers

rep_origin 66..583

/note="CEN/ARS"

/note="CEN6/ARSH4"

/label=CEN/ARS

misc_feature complement(2976..5337)

/label=Ade2

CDS 715..1572

/codon_start=1

/note="Amp"

/note="Ampicillin"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY

I

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY

S

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR

W

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS

A

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA

S LIKHW"

/label=Amp

rep_origin 2280..2520

/note="colE1 ori"

/note="colE1 (pUC18) rep origin"

/label=colE1 ori

misc_feature complement(join(4096..4465,4475..4480,4489..4733,

4735..4737))

/label=MiniDs

CDS 6195..6284

/codon_start=1

/note="GAL4-NLS"

/note="GAL4 NLS"

/translation="MPIDQEKLAKLQKLSANNKVGGTRRKLNKF"

/label=GAL4-NLS

CDS 6285..8399

/codon_start=1

/note="TPase(103-807)4x"

/note="TPase(103-807)"

/translation="MAIVHEPQPQPQPQPEPQPQPQPEPEEEAPQKRAKKCTSDVWQH

F

TKKEIEVEVDGKKYVQVWGHCNFPNCKAKYRAEGHHGTSGFRNHLRTSHSLVKGQLCL

K

SEKDHGKDINLIEPYKYDEVVSLKKLHLAIIMHEYPFNIVEHAYFVEFVKSLRPHFPI

K

SRVTARKYIMDLYLEEKEKLYGKLKDVQSRFSTTMDMWTSCQNKSYMCVTIHWIDDDW

C

LQKRIVGFFHVAGRHTGQRLSQTFTAIMVKWNIEKKLFALSLDNASANEVAVHDIIED

L

QDTDSNLVCDGAFFHVRCACHILNLVAKDGLAVIAGTIEKIKAIVLAVKSSPLQWEEL

M

KCASECDLDKSKGISYAVSTRWNSTYLMLRDALYYKPALIRLKTSDPRRYDAICPKAE

E

WKMALTLFKCLKKFFDLTELLSGTQYSTANLFYKGFCEIKDLIAQWCVHEKFVIRRMA

V

AMSEKFEKYWKVSNIALAVACFLDPRYKKILIEFYMKKFHGDSYKVHVDDFVRVIRKL

Y

QFYSSCSPSAPKTKTTTNDSMDDTLMENEDDEFQNYLHELKDYDQVESNELDKYMSEP

L

LKHSGQFDILSWWRGRVAEYPILTQIARDVLAIQVSTVASESAFSAGGRVVDPYRNRL

G

SEIVEALICTKDWVAASRKGATYFPTMIGDLEVLDSVIAAATNHENHMDEDEDAIEFS

K NNEDVASGSSP"

/label=TPase(103-807)4x

repeat_region 4723..4733

/note="imperfect direct repeat A'"

/label=imperfect direct repeat A'

misc_feature 6723..6725

/note="E249A"

/note="E249A"

/label=E249A

repeat_region 4096..4106

/note="imperfect inverted repeat A"

/label=imperfect inverted repeat A

misc_feature 6724..6724

/note="mutation A to C"

/label=mutation A to C

misc_feature 6984..6986

/note="E336A"

/note="E336A"

/label=E336A

misc_feature 6985..6985

/note="mutation A to C"

/label=mutation A to C(1)

primer_bind complement(4153..4172)

/label=P5_MiniDs

misc_feature 7353..7355

/note="D459A"

/note="D459A"

/label=D459A

misc_feature 7354..7354

/note="mutation A to C"

/label=mutation A to C(2)

misc_feature 7611..7613

/note="D545A"

/note="D545A"

/label=D545A

misc_feature 7612..7612

/note="mutation A to C"

/label=mutation A to C(3)

terminator 8622..8885

/note="CYC1 Term."

/note="KpnI-CYC1-XhoI(sequenced)"

/label=CYC1 Term.

gene complement(9591..10702)

/note="Ura3 gene"

/label=Ura3 gene

CDS complement(9672..10472)

/codon_start=1

/note="URA3"

/note="URA3"

/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL

L

ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKL

Q

YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTG

E

YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVD

D VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"

/label=URA3

promoter 5740..6182

/note="GALS Pr."

/note="Xba I - GALS Promoter - Sac I"

/label=GALS Pr.(1)

misc_feature complement(4092..4094)

/label=MiniDs(1)

misc_feature 4196..4215

/label=MiniDs_P7

primer_bind complement(4096..4129)

/label=688_minidsSEQ1210

misc_feature 4238..4241

/label=DpnII

misc_feature 4300..4303

/label=NlaIII

ORIGIN

1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt

61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata

121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt

181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa

241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata

301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct

361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata

421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact

481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa

541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg

601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg

661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt

721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt

781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg

841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa

901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt

961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag

1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt

1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga

1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt

1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta

1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg

1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc

1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt

1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg

1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg

1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa

1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa

1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga

1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg

1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact

1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac

1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg

1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg

2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga

2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc

2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg

2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc

2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc

2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt

2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc

2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc

2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac

2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact

2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg

2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt

2761 aaccctcact aaagggaaca aaagctggag ctcTTACTGG ATATGTATGT ATGTATAATA

2821 AGTGATCTTA TGTATGAAAT TCTTAAAAAA GGACACCTGT AAGCGTTGAT TTCTATGTAT

2881 GAAGTCCACA TTTGATGTAA TCATAACAAA GCCTAAAAAA TAGGTATATC ATTTTATAAT

2941 TATTTGCTGT ACAAGTATAT CAATAAACTT ATATATTACT TGTTTTCTAG ATAAGCTTCG

3001 TAACCGACAG TTTCTAACTT TTGTGCTTTG ACAAGAACTT CTTCTTCTTG CTTTAATAAA

3061 AACTGTTCCA TTTTCGTTGT ATAACTTGAA TCATAAGCGC CAAGCAGTCT GACAGCCAAC

3121 AGCGCAGCGT TCGTACTATT ATTAATAGCG ACGGTAGCTA CTGGAACACC TCTAGGCATT

3181 TGCACAATTG AATGTAAAGA ATCTACTCCA TCTAGACAAG AACCTTTTAC GGGCACACCG

3241 ATGACAGGAA GTGGTGTCAT TGCAGCCACC ATACCTGGCA AGTGAGCAGC CCCACCAGCT

3301 CCAGCGATAA TTGTTTTAAT TCCACGCTTG CTTGCGGAAA TAGCATATGC TGACATCCTA

3361 TGTGGAGTTC TATGAGCAGA GACTATTGTC ACTTCAAATG GAACGCCAAA ATCTTTTAAA

3421 ACCGCACATG CGGCAGACAT TACCGGCAAG TCAGAGTCTG ATCCCATGAT GATTCCAACC

3481 AATGGTTTGA CCATTGCTTC CAAGTCCAAC TTTTGAGCGA CAGAGATTTT GATTGGAATA

3541 TCAGTTCTAC CTGTAATGTA GTTCAGCCTT TGTTCACATT CCGCCATACT GGAGGCAATA

3601 ATATTTATGT GACCTACTTT TCTGTTAGGT CTAGACTCTT TTCCATATAA GTACACTGAG

3661 GAACCTGGAG TCGCCAATGC TCTTTCGCAA GTTTCTAGCT CTTTATCTTT TGTATGTTTG

3721 TCTCCAAGAA CATTTAGCAT AATGGCGTTC GTTGTAATGG TGGAGAAAGA TGTGAAATTC

3781 TTTGGCATTG GCAAATCCAA TATTGATCTC AAATGAGCTT CAAATTGAGA AGTGACGCAA

3841 GCATCAATGG TATAATGTCC AGAGTTGTGA GGCCTTGGGG CAATTTCGTT AATAAGCAAT

3901 TCCCCTGTTT CTAAATAGAA CATTTCCACA CCAAATATAC CACAACCGGG AAAAGATTTG

3961 ATTGCATTTT CTGCCAACAA CTTCGCCTTA AGTTGAACGG AGTCCGGAAC TCTAGCAGGC

4021 GCATAACATA AGTCACAAAT ATTGTCCTTG TGGATAGTCT CTACAATTGG GTAAGAAAAC

4081 ACTAAACCGT Tgacataggg atgaaaacgg tcggtaacgg tcggtaaaat acctctaccg

4141 ttttcatttt catatttaac ttgcgggacg gaaacgaaaa cgggatatac cggtaacgaa

4201 aacgaacggg ataaatacgg taatcgaaaa ccgatacgat ccggtcgggt taaagtcgaa

4261 atcggacggg aaccggtatt tttgttcggt aaaatcacac atgaaaacat atattcaaaa

4321 cttaaaaaca aatataaaaa attgtaaaca caagtcttaa ttaaacatag ataaaatcca

4381 tataaatctg gagcacacat agtttaatgt agcacataag tgataagtct tgggctcttg

4441 gctaacataa gaagccatat aagtccctcg aggcCTCGAG ggatctgttg cagtcatccc

4501 gaattagaaa atacggtaac gaaacgggat catcccgatt aaaaacggga tcccggtgaa

4561 acggtcggga aactagctct accgtttccg tttccgttta ccgttttgta tatcccgttt

4621 ccgttccgtt ttcgtttttt acctcgggtt cgaaatcgat cgggataaaa ctaacaaaat

4681 cggttatacg ataacggtcg gtacgggatt ttcccatcct actttcatcc ctgcgtcAAC

4741 AGATCTCACA ATCATGACTG CTAATTCTTT AGTAAATGGT GCCCATTTTT CGGCGTACAA

4801 AGGACGATCC TTCAGTACTT CCAAAGCTTC CGGAATCATT TCCTTATTCT TTACAACGAA

4861 GTTACCTCTT CCATCGTATG CCAAAGTCCT CGACTTCAAG ACGAATGGAA AACCCAAATC

4921 TCTTCCAACA TTCAATAGGG ACGTCTCACT GGCTTGTTCC ACAGGAACAC TTTGGGTAAC

4981 TGCTATACCA TTTTTGATTA AATGCTCTTT TTGAATATAT TTGTCTTGTA TCAATCTGAT

5041 TGTTTCTGGA GAAGGGTAAA TTTTTAATTT GGGATGTTTT ACTTGAAGAT TCTTTAGTGT

5101 AGGAACATCA ACATGCTCAA TCTCAATCGT TAGCACATCA CATTTTTCAG CTAGTTTTTC

5161 GATATCAAGA GGATTGGAAA AGGAGCCATT AACGTGGTCA TTGGAGTTGC TTATTTGTTT

5221 GGCAGGAGAA TTTTCAGCAT CTAGTATTAC CGTCTTAATG TTGAGCCTGT TTGCTGCCTC

5281 AACAATCATA CGTCCCAATT GTCCCCCTCC TAATATACCA ACTGTTCTAG AATCCATACT

5341 TGATTGTTTT GTCCGATTTT CTTGTTTTTC TTGATTGTTA TAGTAGGATG TACTTAGAAG

5401 AGAGATCCAA CGATTTTACG CACCAATTTA TACATGAAAT GCTCCATAAT ATTGTCCATT

5461 TAGTTCTTAA TAAAAGGTCA GCAAGAGTCA ATCACTTAGT ATTACCCGGT TCGTAGCCAT

5521 GCAACAAGAG TCATTTGTCA GCATAGCTGT AATAATCAAT CATGACGTAA GAAATGTATC

5581 ATAATTAAAA GTTGTTAAAG ATGTCAGTGT TATGTTGGTG TTACAAAATT CTCGGCTTCT

5641 CACTAATATT TAATATCTCT TAAATTTTAT CTGTCTTTGA TTCTTTTAAG AAAAGTTATG

5701 TATTATTCAA GAAAAAGTCA ATTCCGCATC AAAAGGgagc tcgtgacagc cctccgaagg

5761 aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc

5821 tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag

5881 aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa

5941 ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa

6001 gcgatgattt ttgatctatt aacggatata taaatgcaaa aactgcataa ccactttaac

6061 taatactttc aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa

6121 caaaaaattg ttaatatacc tctatacttt aacgtcaagg agaaaaaacc ccggattcta

6181 gaactagtgg atccatgcca attgaccaag aaaaattagc taagctacaa aagttgtctg

6241 ctaacaacaa agttggtggt actagaagaa agcttaacaa gttcatggct attgttcatg

6301 aaccacaacc acaaccacaa ccacaaccag aaccacaacc acagccacaa cctgaacccg

6361 aagaagaagc accacagaag agggcaaaga agtgcacatc ggatgtatgg cagcatttca

6421 ccaagaagga aattgaagtg gaggtcgatg gaaagaaata cgttcaggta tggggacatt

6481 gcaactttcc taattgcaag gctaagtata gggctgaggg tcatcatgga acaagcggat

6541 ttcgaaatca cttgagaaca tcacatagtt tagttaaagg tcagttgtgt ctaaaaagtg

6601 aaaaggatca tggcaaagac ataaatctca ttgagcctta taagtacgat gaagtggtta

6661 gcctaaagaa gcttcatttg gcaataatca tgcatgaata tcctttcaat attgtagaac

6721 atgcgtactt tgttgagttt gttaagtctc tgcgccctca ctttccaata aagtcccgtg

6781 tcactgctag aaaatatatc atggatttgt atttggaaga aaaagaaaag ttgtatggaa

6841 aactaaaaga tgttcagtct cgcttcagta caactatgga tatgtggaca tcttgtcaaa

6901 ataagtcata catgtgtgtc accatccatt ggattgatga tgattggtgt ctccaaaaaa

6961 gaattgttgg cttttttcat gttgcagggc gccacactgg ccaaaggtta tcacaaacct

7021 tcactgcaat catggttaag tggaacattg agaaaaaatt gtttgccttg tctttggata

7081 atgctagtgc aaatgaagta gctgtgcacg atataattga ggatttgcag gacactgatt

7141 caaatctagt ttgtgatggt gctttctttc atgtgaggtg tgcttgtcac atactgaact

7201 tggttgcaaa ggatggcttg gctgtaattg caggaacaat tgagaaaatc aaagcgattg

7261 ttcttgctgt aaaatcttct cctttgcagt gggaagaact aatgaagtgt gctagtgaat

7321 gtgacttgga taaatctaaa gggatctcat atgctgtctc aactagatgg aattcaacct

7381 atttgatgtt gagggatgcc ttatattata agcctgcact aataaggctt aaaacaagtg

7441 atcctcgcag gtacgatgca atttgtccta aagccgagga gtggaagatg gcattaactc

7501 tttttaagtg tttgaagaag ttttttgatc tcactgaact cctatctggt actcaatatt

7561 ccactgcaaa tttattttac aaaggtttct gtgagataaa ggatttgatt gcccaatggt

7621 gtgttcatga aaaatttgtc attaggagaa tggccgttgc aatgagtgaa aagtttgaga

7681 aatattggaa agtgtctaat attgcactag ctgtagcatg cttccttgac cctaggtaca

7741 agaaaatatt gattgagttc tatatgaaaa aatttcatgg tgattcatac aaagttcatg

7801 tagatgactt tgttagggtc attagaaaat tgtatcaatt ctattctagt tgtagtcctt

7861 cagctccaaa gacaaagaca actactaatg atagtatgga tgataccttg atggaaaatg

7921 aagatgatga atttcaaaac tatttgcatg agttgaagga ttatgatcaa gtagagtcaa

7981 atgaattgga taaatatatg tctgaacccc ttttgaagca tagtggtcag tttgatattt

8041 tatcatggtg gaggggaagg gttgcagaat atcctattct cacccaaatt gcaagggatg

8101 tgctagcaat acaagtgtca actgttgctt ctgagtctgc gttcagtgct ggtggtcgtg

8161 ttgttgatcc ttaccgcaat cgtcttggtt cggagattgt tgaagctttg atatgcacaa

8221 aagattgggt agcagcatct agaaaaggtg ctacatattt tccaacaatg attggtgatc

8281 tcgaggtgct agactctgtt attgctgctg caacaaatca tgagaatcat atggatgagg

8341 atgaagacgc aatagaattt tctaagaata atgaagatgt agcaagtggc tcctctccat

8401 gagcaatgtg tcttatgttt gttgacagat gagccttggt tgtaatagtt tatgcatgct

8461 aagtgatcca gatgtgagca agtgattatg aatatgtgtt ttaaacttta tattgtgtca

8521 tgtgtgctag tagacttata tggcttctta tgttagccaa gggggccccg ggatcccccg

8581 ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagtca tgtaattagt

8641 tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt

8701 tagacaacct gaagtctagg tccctattta tttttttata gttatgttag tattaagaac

8761 gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat

8821 tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcggccg

8881 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa

8941 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct

9001 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc

9061 agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg

9121 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct

9181 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg

9241 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag

9301 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg

9361 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc

9421 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat

9481 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttcc

9541 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata gggtaataac

9601 tgatataatt aaattgaagc tctaatttgt gagtttagta tacatgcatt tacttataat

9661 acagtttttt agttttgctg gccgcatctt ctcaaatatg cttcccagcc tgcttttctg

9721 taacgttcac cctctacctt agcatccctt ccctttgcaa atagtcctct tccaacaata

9781 ataatgtcag atcctgtaga gaccacatca tccacggttc tatactgttg acccaatgcg

9841 tctcccttgt catctaaacc cacaccgggt gtcataatca accaatcgta accttcatct

9901 cttccaccca tgtctctttg agcaataaag ccgataacaa aatctttgtc gctcttcgca

9961 atgtcaacag tacccttagt atattctcca gtagataggg agcccttgca tgacaattct

10021 gctaacatca aaaggcctct aggttccttt gttacttctt ctgccgcctg cttcaaaccg

10081 ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt

10141 ctgtatacac ccgcagagta ctgcaatttg actgtattac caatgtcagc aaattttctg

10201 tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct ttagcggctt aactgtgccc

10261 tccatggaaa aatcagtcaa gatatccaca tgtgttttta gtaaacaaat tttgggacct

10321 aatgcttcaa ctaactccag taattccttg gtggtacgaa catccaatga agcacacaag

10381 tttgtttgct tttcgtgcat gatattaaat agcttggcag caacaggact aggatgagta

10441 gcagcacgtt ccttatatgt agctttcgac atgatttatc ttcgtttcct gcaggttttt

10501 gttctgtgca gttgggttaa gaatactggg caatttcatg tttcttcaac actacatatg

10561 cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt

10621 accgaatcaa aaaaatttca aagaaaccga aatcaaaaaa aagaataaaa aaaaaatgat

10681 gaattgaatt gaaaagctgt ggtatggtgc actctcagta caatctgctc tgatgccgca

10741 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg

10801 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg

10861 ttttcaccgt catcaccgaa acgcgcga

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